Hb estimation methods

[David.]

Hb estimation methods include all except:
a) Drabkins method
b) Wintrobes method
c) Sahli’s method
d) Spectrophotometry

[David.]

Ans. b) Wintrobes method (used for ESR)
Drabkin's solution containing potassium ferricyanide, potassium cyanide and sodium bicarbonate was prepared. 20 microliter of blood was added to 5ml of Drabkin's solution. Readings were taken at 530 nm in a spectrophotometer. Haemoglobin values were calculated from a haemoglobin curve prepared using haemoglobin standard.